Cytelligen
Products for Culture and Freezing of Human Primary Tumor Cells

Cytelligen has developed a novel technology and related products suitable for culture and/or freezing of human primary tumor cells enriched from patients' blood, ascites, pleural effusion, bone marrow, urine, etc. Tumor cells in bio-fluid samples saved in the reagents containing special anti-coagulants (FCH-001) are enriched using SET series subtraction enrichment products. Enriched tumor cells with appropriate amount and ratio of hematopoietic feeder cells, free from hypotonic damage, are immediately added into the following special cell culture media for primary tumor cell culture. Human primary tumor cells are selected to grow more while hematopoietic cells (such as WBCs) will be gradually depleted during cell culture. Cultured primary tumor cells are available for either freezing in the special primary cell freezing media provided in the FCH-001 product or downstream biological and genetic analyses as well as establishing CTC or CTC subtype derived xenograft (CDX) mouse models.

If freezing of tumor cells rather than cell culture is immediately desired, bio-fluid samples could be processed using the reagents provided in the FCH-001 product, followed by freezing in the special freezing media suitable for primary tumor cells (FCH-001). However, one  has to perform enrichment on thawed cells prior to further primary tumor cell culture or downstream biological and genetic analyses, etc.  

 

Choice 1: samples mixed with anti-coagulant (FCH-001) - enrichment (SEH product) - primary tumor cell culture (FCH-002)

- either downstream analysis or freezing (FCH-001).

Choice 2: samples mixed with anti-coagulant (FCH-001) - processing (FCH-001) - freezing (FCH-001) - thawing and

enrichment (FCH-003 product) - cell culture (FCH-002) - downstream analysis.

Product Name

Catalog No.

Tests/Kit

Human Primary Tumor Cell Treatment and Freezing Kit
(suitable for human blood, pleural effusion, ascites, bone marrow and urine sample, etc.)

FCH-001A

2

FCH-001B

10

FCH-001C

50

FCH-001D

100

Human Primary Tumor Cell Culture Medium
(including all the essential supplements)  

FCH-002

500 ml/bottle

Post-Freezing Enrichment Kit for Human Primary Tumor Cells
(suitable for human blood, pleural effusion, ascites, bone marrow and urine sample, etc.)

FCH-003A

2

FCH-003B

10

FCH-003C

50

FCH-003D

100

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