Cytelligen has developed a novel technology and related products suitable for culture and/or freezing of human primary tumor cells enriched from mouse blood. Tumor cells in mouse blood samples saved in the reagents containing special anti-coagulants (FCM-001) are enriched using SEM series subtraction enrichment products. Enriched tumor cells, free from hypotonic damage, are immediately added into the following special cell culture media for primary tumor cell culture. Human primary tumor cells are selected to grow more while mouse hematopoietic cells (such as WBCs) will be gradually depleted during cell culture. Cultured primary tumor cells are available for either freezing in the special primary cell freezing media provided in the FCM-001 product or downstream biological and genetic analyses.
If freezing of tumor cells rather than cell culture is immediately desired, mouse blood samples could be processed using the reagents provided in the FCM-001 product, followed by freezing in the special freezing media suitable for primary tumor cells (FCM-001). However, one has to perform enrichment (SEM series products) on thawed cells prior to further primary tumor cell culture or downstream biological and genetic analyses, etc.
Choice 1: samples mixed with anti-coagulant (FCM-001) - enrichment (SEM product) - primary tumor cell culture (FCH-002)