Necrotic cell has compromised plasma membrane and nuclear membrane, results in its nucleus being stained by specialized reagents. Co-staining of necrotic CTC with antibodies against additional markers of epithelial cell or other cell type is performed simultaneously. In addition to immunostaining alone, a novel Necro-iFISH technology specialized in identifying necrotic CTC is also available.
Identification of necrotic CTC helps rapidly evaluate therapeutic efficacy of anti-cancer drugs on tumor cells in real time.
Immunofluorescence staining of human CTC enriched from mouse blood in liquid phase is easy, rapid. Identified cell which has brighter fluorescence intensity is useful for enumeration of human CTC in mouse sample.