Z. Fu, L. Zhang, R. Chen, J. Zhan, J. Zhong, W. Zheng, J. Zou, P. Wang, X. Deng, A.Y. Lin, D.D. Wang, P. P. Lin, R. He (2024) Biphasic co-detection of melanoma aneuploid tumor cells and tumor endothelial cells in guidance of specifying the field cancerized surgical excision margin and administering immunotherapy. Cancer Letters 598:217099
Key words: iFISH tumor tissue and liquid biopsies, field cancerized excision margin, melanoma, immunotherapy, cellular MRDQ. Sun, W. Li, D. Yang, P.P. Lin, L. Zhang, H. Guo (2024) The Presence of Small-Size Circulating Tumor Cells Predicts Worse Prognosis in Non–Small Cell Lung Cancer Patients. Arch Pathol Lab Med doi 10.5858/arpa.2023-0455-OA
Key words: small cell sized CD31- aneuploid CTC, large cell sized CD31+ aneuploid CTEC, NSCLC, prognosisS. Li, S. Luo, N. Wei, A.Y. Lin, D.D. Wang, P.P. Lin, R. Chen, J. Xie, and J. Ren (2023) A rare case of Merkel cell carcinoma on the craniofacial region and characterization of its aneuploid CD31- CTCs and CD31+ CTECs expressing EpCAM or Ki-67. Oral Oncol 147:106602
Key words: skin cancer、Ki67+ CTC/CTEC、SE-iFISHY. Wang, L. Zhang, J. Tan, Z. Zhang, Y. Liu, X. Hu, B. Lu, Yuan Gao, L. Tong, Z. Liu, H. Zhang, P. P. Lin, B. Li, O. Gires, T. Zhang (2023) Longitudinal detection of subcategorized CD44v6+ CTCs and circulating tumor endothelial cells (CTECs) enables novel clinical stratification and improves prognostic prediction of small cell lung cancer: a prospective, multi-center study. Cancer Lett 571:216337
Key words: SCLC, stem cell marker CD44v6, CD44v6+ CTCs and CTECs, brain and distant metastases, stratification, prognosisY. Wang, Y. Liu, Z. Zhang, B. Lu, Y. Gao, L. Tong, M. Hu, P. P. Lin, B. Li and T. Zhang. (2023) Post-therapeutic circulating tumor cell-associated white blood cell clusters predict poor survival in patients with advanced driver gene-negative non-small cell lung cancer. BMC Cancer 23:578
Key words: Driver-gene mutation negative NSCLC, CTC-WBC, SE-iFISHJ. Mi, F. Yang, J. Liu, M. Liu, A. Y. Lin, D. D. Wang, P. P. Lin and Q. Zeng (2022) Case Report: Post-therapeutic Laryngocarcinoma Patient Possessing a High Ratio of Aneuploid CTECs to CTCs Rapidly Developed de novo Malignancy in Pancreas. Front Oncol 12:981907
Key words: MRD, prediction of cancer occurrence, liquid biopsy, aneuploid CTCs and CTECs, SE-iFISHM. Li, F. Gao, X, Ren, G. Dong, H. Chen, A.Y. Lin, D.D. Wang, M. Liu, P.P. Lin, S. Shen, H. Jiang, C. Yang, X. Zhang, X. Zhao, Q. Zhu, M. Li, Y Cui, S. Lin (2022) Nonhematogenic circulating aneuploid cells confer inferior prognosis and therapeutic resistance in gliomas. Cancer Sci 113:3535–3546
Key words: blood–brain-barrier, CD31- CTC, CD31+ CTEC, glioma, prognosis, therapy resistanceZ. Li, L. Fan, Y. Wu, Y. Niu, X. Zhang, B. Wang, Y. Yao, C. Chen, N. Qi, D. D. Wang, P. P. Lin, D. Tang, W. Gao (2022) Analysis of the prognostic role and biological characteristics of circulating tumor cell-associated white blood cell clusters in non-small cell lung cancer. J Thorac Dis 14:1544-1555
Key words: CTC-WBC clusters, inferior prognosis, NSCLCA.Y. Lin, D.D. Wang, L. Li and P.P. Lin (2021) Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood. Cancers 13:5108.
Key words: necrotic CTCs and CTECs, rapid evaluation of therapeutic effects, MRD; liquid biopsy, SE-iFISH (NC)T. Zhang, L. Zhang, Y. Gao, Y. Wang, Y. Liu, H. Zhang, Q. Wang, F. Hu, J. Li, J. Tan, D.D. Wang, O. Gires, P.P. Lin and B. Li (2021) Role of aneuploid circulating tumor cells and CD31+ circulating tumor endothelial cells in predicting and monitoring anti-angiogenic therapy efficacy in advanced NSCLC. Mol Oncol 15:2891-2909
Key words: bevacizumab, aneuploid CTC and CTEC, EMT and EndoMT, NSCLCY. Chen, Y. Li, C. Qi, C. Zhang, D. Liu, Y. Deng, Y. Fu, V.S. Khadka, D.D. Wang, S. Tan, S. Liu, Z. Peng, J. Gong, P.P. Lin, X. Zhang, J. Li, Y. Li, L. Shen (2021) Dysregulated KRAS gene-signaling axis and abnormal chromatin remodeling drive therapeutic resistance in heterogeneous-sized circulating tumor cells in gastric cancer patients. Cancer Lett 517:78-87
Key words: single cell NGS, large and small iFISH CTCs, gastric cancer, prognosis, therapy resistanceP.P. Lin (2021) Chapter: Liquid Biopsy Analysis of Circulating Tumor Biomarkers in Lung Cancer, in the book “Lung Cancer – Modern Multidisciplinary Management” edited by Henry S. Park, Yale University School of Medicine; published by IntechOpen Publisher, London, UK.
Key words: liquid biopsy, lung cancer, CTC and CTEC, SE-iFISHC. Xing, Y. Li, C. Ding, S. Wang, H. Zhang, L. Chen, P Li, M. Dai (2021) CD44+ Circulating Tumor Endothelial Cells Indicate Poor Prognosis in Pancreatic Ductal Adenocarcinoma After Radical Surgery: A Pilot Study. Cancer Manag Res 13:4417-4431
Key words: pancreatic cancer, post-surgical early recurrence, CD44+ CTECs and CTCs, SE-iFISHY.Hong, J. Si, J. Zhang, Y. Xiong, J. Zhang, P. Lin, J. Fang, Y. Yang, C. Lv and Y. Ma (2021) Small Cell Size Circulating Aneuploid Cells as a Biomarker of Prognosis in Resectable Non-Small Cell Lung Cancer. Front Oncol 11:590952
Key words: early stage lung cancer, pre-surgical small cell CTCs, prognosisP. P. Lin (2020) Aneuploid Circulating Tumor-Derived Endothelial Cell (CTEC): A Novel Versatile Player in Tumor Neovascularization and Cancer Metastasis (Review). Cells 9:1539
Key words: endothelialization of cancer cells, tumor microenvironment, hypoxia, EMT and EndoMT, transdifferentiation, cell fusionL. Zhang, X. Zhang, Y. Liu, T. Zhang, Z. Wang, M. Gu, Y. Li, D.D. Wang, W. Li, P.P. Lin (2020) PD-L1+ aneuploid circulating tumor endothelial cells (CTECs) exhibit resistance to the checkpoint blockade immunotherapy in advanced NSCLC patients. Cancer Lett 469:355-366
Key words: NSCLC, PD-L1, CTEC and CTC, resistance to immunotherapyY. Zhao, J. Li, D. Li, Z. Wang, J. Zhao, X. Wu, Q. Sun, P.P. Lin, P. Plum, A. Damanakis, F. Gebauer, M. Zhou, Z. Zhang, H. Schlosser, K-W Jauch, P.J. Nelson, C.J. Bruns (2020) Tumor biology and multidisciplinary strategies of oligometastasis in gastrointestinal cancers (Review). Semin Cancer Biol 60:334-343
Key words: multiple carcinomas, CTECY. Lei, N. Sun, G. Zhang, C. Liu, Z. Lu, J. Huang, C. Zhang, R. Zang, Y. Che, S. Mao, L. Fang, X. Wang, S. Zheng, J. He (2020) Combined detection of aneuploid circulating tumor-derived endothelial cells and circulating tumor cells may improve diagnosis of early stage non-small-cell lung cancer. Clin Transl Med e128
Key words: NSCLC, early diagnosis, aneuploid CTEC and CTC, VimentinG. Ma, Y. Jiang, M. Liang, J. Li, J. Wang, X. Mao, J. S. Veeramootoo, T. Xia, X. Liu, and S. Wang (2020) Dynamic monitoring of CD45-/CD31+/DAPI+ circulating endothelial cells aneuploid for chromosome 8 during neoadjuvant chemotherapy in locally advanced breast cancer. Ther Adv Med Oncol 12:1-14
Key words: breast cancer, EpCAM+ CTEC and CTC, neoadjuvant chemotherapyX. Liu, J. Li, B. L. Cadilha, A. Markota, C. Voigt, Z. Huang, P. P. Lin, D. D. Wang, J. Dai, G. Kranz, A. Krandick, D. Libl, H. Zitzelsberger, I. Zagorski, H. Braselmann, M. Pan, S. Zhu,Y. Huang, S. Niedermeyer, C. A. Reichel, B. Uhl, D. Briukhovetska, J. Suárez, S. Kobold, O. Gires, and H. Wang (2019) Epithelial-type systemic breast carcinoma cells with a restricted mesenchymal transition are a major source of metastasis (SA Highlight). Sci Adv 5, eaav4275
Key words: breast cancer, CTC, systemic metastasisY. Wang, Y. Liu, L. Zhang, L. Tong, Y. Gao, F. Hu, P. P. Lin, B. Li, T. Zhang (2019) Vimentin expression in circulating tumor cells (CTCs) associated with liver metastases predicts poor progression‑free survival in patients with advanced lung cancer. J Cancer Res Clin Oncol 145:2911-2920
Key words: lung cancer, Vimentin+ CTC, hepatic metastasisZ. Ye, Y. Ding, Z. Chen, Z. Li, S. Ma, Z. Xu, L. Cheng, X. Wang, X. Zhang, N. Ding, Q. Zhang, and Q. Qian (2019) Detecting and phenotyping of aneuploid circulating tumor cells in patients with various malignancies. Cancer Biol Ther 20:546
Key words: multiple types of carcinomas, small cell sized mesenchymal CTCs, SE-iFISHY. Zhang, J. Li, Li. Wang, P. Meng, J. Zhao, P. Hang, J. Xia, J. Xu, Li. Wang, F. Shen, A. Zheng, F. Zhou and R. Fan (2019) Clinical significance of detecting circulating tumor cells in patients with esophageal squamous cell carcinoma by EpCAM-independent enrichment and immunostaining-fluorescence in situ hybridization. Mol Med Rep 20:1551
Key words: esophageal cancer, adjuvant therapy, post-surgical detection, prognosisJ. Zhang, H. Shi, T. Jiang, Z. Liu, P.P. Lin, and N. Chen (2018) Circulating tumor cells with karyotyping as a novel biomarker for diagnosis and treatment of nasopharyngeal carcinoma. BMC Cancer 18:1133
Key words: nasopharyngeal carcinoma, EpCAM+ CTCY. Li, X. Zhang, D. Liu, J. Gong, D. Wang, S. Li, Z. Peng, Y. Li, X. Wang, P. Lin, M. Li, and L. Shen (2018) Evolutionary expression of HER2 conferred by chromosome aneuploidy on circulating gastric cancer cells contributes to developing targeted and chemotherapeutic resistance (CCR Highlight). Clin Cancer Res 24:5261-71
Key words: gastric cancer, HER2+ CTC, intrinsic and acquired chemoresistanceP.P. Lin (2018) Aneuploid CTC and CEC (Review). Diagnostics (Basel) 8(2):26
Key words: CTEC and CTC, SE-iFISH, ALK-iFISHL. Wang, Y. Li, J. Xu, A. Zhang, X. Wang, R. Tang, X. Zhang, H. Yin, M. Liu, D. Wang, P. Lin, L. Shen, J. Dong (2018) Quantified postsurgical small cell size CTCs and EpCAM+ circulating tumor stem cells with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse. Cancer Lett 412:99-107
Key words: hepatocellular carcinoma, EpCAM+ CTC, post-surgical recurrenceX. Li, Y. Zhang, J. Ding, M. Wang, N. Li, H. Yang, K. Wang, D. Wang, P. Lin, M. Li, Z. Zhao, and P. Liu (2018) Clinical significance of detecting CSF-derived tumor cells in breast cancer patients with leptomeningeal metastasis. Oncotarget 9:2705-14
Key words: breast cancer, brain metastasis, CTC in cerebrospinal fluid, NGS, primary tumor cell culture, therapeutic drugs screening in vitroX. Liu, Z. Zhang, B. Zhang, Y. Zheng, C. Zheng, B. Liu, S. Zheng, K. Dong, R. Dong (2018) Circulating tumor cells detection in neuroblastoma patients by EpCAM-independent enrichment and immunostaining-fluorescence in situ hybridization. EBioMedicine 35:244
Key words: neuroblastoma, metastasis, prognosisZ. Su, J. Zhao, S. KE, J. Zhang, X. Liu, Y. Wang, Q. Sun and Q. Pan (2018) Clinical significance of circulating tumor cells via combined whole exome sequencing in early stage cancer screening: A case report. Exp Ther Med 16:2527
Key words: early detection, colorectal cancer, intestinal polyp, whole exome sequencingP.P. Lin, O. Gires, D. Wang, L. Li, and H. Wang (2017) Comprehensive in situ co-detection of aneuploid circulating endothelial and tumor cells. Sci Rep 7:9789
Key words: CTEC and CTC, SE-iFISHH. Wang, N. Stoecklein, P. Lin and O. Gires (2017) Circulating and disseminated tumor cells: diagnostic tools and therapeutic targets in motion (Review). Oncotarget 8:1884-1912
Key words: CTC and DTC, EMTY. Xu, T. Qin, J. Li, X. Wang, C. Gao, C. Xu, J. Hao, J. Liu, S. Gao and H. Ren (2017) Detection of Circulating Tumor Cells Using Negative Enrichment Immunofluorescence and an In Situ Hybridization System in Pancreatic Cancer. Int J Mol Sci 18:622
Key words: pancreatic cancer CTC and CTM, trisomy 8, CA19-9, chemo-sensitivity, prognosis, SE-iFISHW. Wu, Z. Zhang, X. Gao, Z. Shen, Y. Jing, H. Lu, H. Li, X. Yang, X. Cui, Y. Li, Z. Lou, P. Liu, C. Zhang, W. Zhang (2017) Clinical significance of detecting circulating tumor cells in colorectal cancer using subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) Oncotarget 8:21639
Key words: colorectal cancer CTCs, post-surgical recurrence, prognosis, SE-iFISHY. Sheng, T. Wang, H. Li, Z. Zhang, J. Chen, C. He, Y. Li, Y. Lv, J. Zhang, C. Xu, Z. Wang, C. Huang and L. Wang (2017) Comparison of analytic performances of Cellsearch and iFISH approach in detecting circulating tumor cells. Oncotarget 8:8801
Key words: breast cancer, comparison of CellSearch vs SE-iFISHY. Gao, Y. Zhu, Z. Zhang, C. Zhang, X. Huang and Z. Yuan (2016) Clinical significance of pancreatic circulating tumor cells using combined negative enrichment and immunostaining-fluorescence in situ hybridization. J Exp Clin Cancer Res 35:66
Key words: pancreatic cancer, different stages, prognosisF. Gao, Y. Cui, H. Jiang, D. Sui, Y. Wang, Z. Jiang, J. Zhao, S. Lin (2016) Circulating tumor cell is a common property of brain glioma and promotes the monitoring system. Oncotarget 7:71330-71340
Key words: brain glioblastoma, CTCY.Li, X. Zhang, J. Gong, Q. Zhang, J. Gao, Y. Cao, D. Wang, P. Lin, L. Shen (2016) Aneuploidy of chromosome 8 in circulating tumor cells correlates with prognosis in patients with advanced gastric cancer. Chin J Cancer Res 28:579-588
Key words: gastric cancer, CTC, chemoresistance, prognosisP. Lin (2015) Integrated EpCAM-independent subtraction enrichment and iFISH strategies to detect and classify disseminated and circulating tumors cells (Review). Clin Transl Med 4(38):1-7
Key words: SE-iFISH, CTCJ. Jiang, D. Wang, M. Yang, D. Chen, L. Pang, S. Guo, J. Cai, J. Wery, L. Li, H. Li, P. Lin (2015) Comprehensive characterization of chemotherapeutic efficacy on metastases in the established gastric neuroendocrine cancer patient derived xenograft model. Oncotarget 6:15639-15651
Key words: gastric cancer, metastatic PDX, CK18+ CTC, susceptibility and resistance to chemotherapyF. Ge, H. Zhang, D. Wang, L. Li and P. Lin (2015) Enhanced detection and comprehensive in situ phenotypic identification of circulating and disseminated heteroploid epithelial and glioma tumor cells. Oncotarget 6: 27049-27064
Key words: SE-iFISH, lung cancer, esophageal carcinoma, glioma, CTC and DTC, cerebrospinal fluidY.Li, X. Zhang, S. Ge, J. Gao, J. Gong, M. Lu, Q. Zhang, Y. Cao, D. Wang, P. Lin, L. Shen (2014) Clinical Significance of Phenotyping and Karyotyping of Circulating Tumor Cells in Patients with Advanced Gastric Cancer. Oncotarget 5:6594-6602
Key words: gastric cancer, CTC, trisomy 8, chemoresistance to cisplatinQ. Chen, F. Ge, W. Cui, F. Wang, Z. Yang, Y. Guo, L. Li, R.M. Bremner, P.P. Lin (2013) Lung cancer circulating tumor cells isolated by the EpCAM-independent enrichment strategy correlate with Cytokeratin 19-derived CYFRA21-1 and pathological staging. Clin Chim Acta 419:57-61
Key words: lung cancer, CTC, Cyfra21-1R. Ran, L. Li, M. Wang, S. Wang, Z. Zheng, and P. Lin (2013) Determination of EGFR mutation in single cells microdissected from enriched lung tumor cells in peripheral blood. Anal Bioanal Chem 405(23):7377-82
Key words: lung cancer, single CTC analysis, EGFR mutation, WGA, real-time PCRC. Wu, H. Hao, L. Li, X. Zhou, Z. Guo, L. Zhang, X. Zhang, W. Zhong, H. Guo, R. Bremner, and P. Lin (2009) Preliminary investigation of the clinical significance of detecting circulating tumor cells enriched from lung cancer patients. J Thorac Oncol 4:30-36
Key words: lung cancer, CTC, prognosisCytelligen, the real solutions for circulating rare cells……